(1) When using an optical microscope, first check whether the overall installation of the microscope is loose, for example,Optical microscopeLong-term use can lead to problems such as loose support frames and overly loose lens installation. Too loose would harm the effectiveness of testing practical tests and easily damage the optical microscope.
(2) Common issues in using the focal screw setup. During operation, it is very easy to focus immediately under a high-magnification lens, or to keep both eyes looking at the eyepiece regardless of whether the barrel is raised or lowered, or to avoid the critical point of controlling the distance. When the distance is set to 2~3 cm, the focus increases further, and the rotation speed of the focus spiral is rapid. These problems easily lead to the objective lens lens being damaged during loading or damaging the mounting or camera lens. Regarding the above practical operation challenges, I want to say that when adjusting the lens focal length, you must land at low magnification. First, rotate the coarse focal length screw to gradually lower the barrel, bringing the objective lens close to the coverslip. Be careful not to let the objective touch the coverslip. Throughout this process, both eyes should look at the objective from the side, then use your right eye to look inside the eyepiece and slowly adjust the coarse focal length in the opposite direction so the barrel slowly rises until you see the virtual image. Also, note 'normal' for studentsOptical microscopeThe object distance is around 1 cm, so if the distance is well exceeded 1 cm but no virtual image is seen, this may be because the specimen collection is not within the field of view or the rotation of the rough focal screw is too fast. In this case, the loading area should be adjusted, and the process should be repeated. When a blurry virtual image appears in the field of view, a fine focal screw adjustment is required. Only in this way can the search range be reduced and the speed of virtual image search improved.
(3) The challenge of optical microscopes regarding light. Lighting is an applicationDuring optical microscopyA very important step, remember, is to use a low-power lens to direct the light. When the light source is strong, use a small focal length or a flat lens; when the light source is weak, use a large aperture lens or concave lens. When rotating the mirror, do not pull it out with one hand; rotate it with both hands until you see a uniform, bright circular field of view. After the light is properly aligned, never move the optical microscope arbitrarily, to prevent the light source from accurately entering the light source through the rearview mirror.
(4) The challenge of objective lens transformation. After using a low-power lens, switching to a high-power lens usually means people prefer to immediately rotate the objective lens with their fingers, thinking this saves effort. However, this easily causes the optical axis to tilt. The reason is that the original material of the converter is too soft in color, has high precision, and the external thread support is uneven, making it very easy for it to loosen. Once the external thread is damaged, the converter will be charged with the full inverter. Therefore, I recommend holding the next layer of the converter, the rotating plate to change the objective lens.
(5) High magnificationOptical microscopeThe challenge of using the eyes. When using a high-power microscope objective, the right eye should be as close as possible to the eyepiece. The right eye should try to look into the field of view, not forcefully covering the right eye or closing it decisively, as this violates experimental observation rules and often causes fatigue. Additionally, it cannot guarantee that the right eye is drawing while observing.
